Tiger Liu

Tiger Liu is the Global Agent at Duebio (the international trade arm of TiosBio), a Chinese IVD manufacturer with 20+ years of experience in molecular diagnostics. He focuses on CRISPR Cas12/Cas13 detection, RAA isothermal amplification, lateral flow assays, microfluidic PCR, TRF immunoassays, and OEM/ODM IVD product development for global distributors. Reach Tiger at medtiger@foxmail.com.

Machine Learning for CRISPR Lateral Flow Interpretation (2025)

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💡 Tech Spotlight: Machine Learning for LFA interpretation. Due Bio’s high-consistency manufacturing ensures the strip-to-strip uniformity required for accurate AI-based diagnostics. Title: Rapid and automated interpretation of CRISPR-Cas13-based lateral flow assay test results using machine learning Source: Sensors & Diagnostics (RSC) (2025) Abstract CRISPR-Cas-based lateral flow assays (LFAs) have emerged as a promising diagnostic tool … Read more

Multiplexed CRISPR Lateral Flow for SARS-CoV-2 (Biosensors & Bioelectronics)

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Title: Multiplexed lateral flow assay integrated with orthogonal CRISPR-Cas system for SARS-CoV-2 detection Source: Biosensors and Bioelectronics (PMC9396441) Abstract The development of field-deployable detection platform amenable for multiplexed genes testing will significantly improve the efficiency and reliability during point-of-care testing (POCT) applications. In this regard, an orthogonal CRISPR-Cas-mediated multiplexed lateral flow assay (designated as OC-MLFA) … Read more

Detection of Bean Pod Mottle Virus (Nature Sci Rep)

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Title: A reverse transcription-cross-priming amplification method with lateral flow dipstick assay for the rapid detection of Bean pod mottle virus Source: Scientific Reports (Nature) Introduction Bean pod mottle virus (BPMV) is a comovirus that has a bipartite positive-strand RNA genome consisting of RNA-1 and RNA-2. It has become one of the most economically important soybean … Read more

Rapid Detection of SARS-CoV-2 using RT-RAA/LFD

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Title: Reverse Transcription Recombinase-Aided Amplification Assay With Lateral Flow Dipstick Assay for Rapid Detection of 2019 Novel Coronavirus Source: Frontiers in Cellular and Infection Microbiology (PMC7882697) Abstract Background: The emerging Coronavirus Disease-2019 (COVID-19) has challenged the public health globally. With the increasing requirement of detection for SARS-CoV-2 outside of the laboratory setting, a rapid and … Read more

CRISPR/Cas12a Detection of Feline Parvovirus

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Title: Rapid detection of feline parvovirus using RAA-CRISPR/Cas12a-based lateral flow strip and fluorescence Source: Frontiers in Microbiology (PMC11932995) Abstract Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats, with high morbidity and mortality, necessitating a rapid and effective antigen diagnostic test with high sensitivity and specificity. In this study, a diagnostic platform based on … Read more

Rapid Detection of Shigella spp. using RPA-LFD

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Title: Development of a recombinase polymerase amplification assay with lateral flow dipstick (RPA-LFD) for rapid detection of Shigella spp. and enteroinvasive Escherichia coli Source: PLOS ONE (PMC9744308) Citation: Zhang L, et al. (2022). Development of a recombinase polymerase amplification assay with lateral flow dipstick (RPA-LFD) for rapid detection of Shigella spp. and enteroinvasive Escherichia coli. … Read more