Cool Flash Nucleic Acid Release Reagent — Cat. No. BT0068 is a sub-minute enzyme-free nucleic acid release reagent for animal and plant samples.
Characteristics
- This reagent can be used for nucleic acid (both DNA and RNA) extraction, enrichment, and purification from
- animal samples including whole blood, serum, plasma, urine, saliva, oral swabs, nasopharyngeal swabs, and cell
- suspensions, or plant samples including roots, stems, leaves, flowers, and seeds. The lysate after nucleic acid release
- reagent treatment can be directly used for PCR amplification with Tiosbio® Marathon DNA Polymerase (BT0016),
- and for isothermal amplification with Tiosbio® RAA Nucleic Acid Amplification Kit (JY0200), Tiosbio® RT RAA
- Nucleic Acid Amplification Kit (JY0203), and Tiosbio® RAA Nucleic Acid Amplification Kit (Lateral Flow Strip
Specifications and Storage
| Item | Details |
|---|---|
| Catalog Number | BT0068 |
| Product Name | Cool Flash Nucleic Acid Release Reagent |
| Operation Time | < 1 minute |
| Storage Conditions | Room temperature |
| Shelf Life | 12 months |
Procedure
1. Animal Samples After appropriate processing of samples such as whole blood, serum, plasma, urine, saliva, oral swabs, nasopharyngeal swabs, and cell suspensions, mix with the sample to be tested at a ratio of Cool Flash Nucleic Acid Release Reagent (BT0068) to liquid sample = 1:7. When the sample concentration is low, it is recommended that the lysis system be ≥ 50 μL to ensure that the lysate contains a detectable amount of nucleic acid sample. After thoroughly pipetting the mixed solution 20 times with a pipette tip or disposable plastic pipette, aspirate the supernatant into a clean centrifuge tube for testing. For PCR, RAA, or RT RAA amplification, the lysate supernatant can be added up to the maximum allowable volume of the reaction system. 2. Plant Samples After simple rinsing of the material to be tested, cut into 2-5 mm square small pieces or segments, add 100 μL of Cool Flash Nucleic Acid Release Reagent (BT0068), and crush the material several times with a pipette tip. After simple crushing of the sample, thoroughly pipette 20 times with a pipette tip or disposable plastic pipette, then aspirate the supernatant into a clean centrifuge tube for testing. For PCR, RAA, or RT RAA amplification, 1 μL of lysate supernatant can be taken for detection. Addition amount of lysate in amplification system: If the detection results of the sample to be tested, positive control, and negative control samples after lysis do not match the actual situation, parallel tests can be set up with a gradient of ±2 μL of lysate addition volume to determine the effect of different lysate volumes on the detection of samples, positive controls, and negative controls within the same amplification system. Under the condition that the positive control detection result is positive and the negative control detection result is negative, select the maximum lysate addition amount. Since Cool Flash Nucleic Acid Release Reagent has the effect of inhibiting non-specific amplification, when all negative controls are detected as negative and all positive controls are detected as positive, the lysate can be added at the maximum sample addition volume allowed by the amplification system.
Warnings and Precautions
- When using this reagent for nucleic acid extraction from whole blood, serum, plasma, urine, saliva, oral swabs,
- nasopharyngeal swabs, or cell suspensions, sample processing should be completed immediately after sample
- collection whenever possible. If conditions do not allow, samples should be transported under refrigerated or frozen
- When used for infectious disease sample testing, pay attention to safety protection during operation.
- Operations should be conducted in designated laboratory facilities. Wear protective clothing, disposable gloves, and
- masks. All items that have directly contacted pathogen samples should be disinfected before disposal or reuse.
- This reagent is designed for rapid crude processing of animal liquid samples. The processed material can be
- used for PCR amplification with Tiosbio® Marathon DNA Polymerase (BT0016), and for isothermal amplification
- with Tiosbio® RAA Nucleic Acid Amplification Kit (JY0200), Tiosbio® RT RAA Nucleic Acid Amplification Kit
FAQ: Cool Flash Nucleic Acid Release Reagent
What is BT0068 used for?
BT0068 is a sub-minute enzyme-free nucleic acid release reagent for animal and plant samples. It is designed for rapid sample processing in molecular diagnostics and research applications.
Does Due Bio support OEM/ODM for BT0068?
Yes. Due Bio / TiosBio supports OEM packaging, private-label formulations, bulk supply, and customized documentation for qualified distributors and diagnostic manufacturers. Contact Due Bio with your target market, expected annual volume, and packaging requirements.
Related Technical Guides
This product is supplied for research use only. For OEM/ODM cooperation, distributor quotations, or regulatory documentation requests, contact Due Bio through the Contact page.