Working Mechanism
This product uses a chromatographic double-antibody sandwich method to quickly detect nucleic acid amplification products. Compared with the traditional agarose gel electrophoresis detection, the nucleic acid detection test strip is simple to operate, quick to interpret, does not contain toxic substances, and does not require any equipment. When designing the first detection sequence primer, the user only needs to label one primer/probe with Biotin and the other primer/probe with FITC or 6-carboxyfluorescein 6-FAM. When designing the second detection sequence primer, label one primer/probe with Rhodamine TAMRA, and label the other primer/probe with Digoxin. There are 2 labeling options to choose from, see the table below. Also, ensure that the two markers can be integrated into the double-stranded amplification product at the same time, then this product can be used for detection. If the internal reference design is used, please design the internal reference fragment marker as digoxin.
Marking options
- 5 ’Biotin—-3’ FAM(FITC),5’ Digoxin—–3’ TAMRA
- 5’ Biotin—– Digoxin ,5’ TAMRA —–3’ FAM(FITC)
FAQ: Double-Labeled Nucleic Acid Detection Test Strip
What is a double-labeled nucleic acid detection strip?
A double-labeled nucleic acid detection strip is designed to read amplification products or assay targets carrying two compatible labels, enabling lateral flow detection of molecular assay products.
What applications are suitable for this strip?
It can be evaluated for molecular assay development, RAA/RPA endpoint detection, LAMP readout, PCR product detection, and custom nucleic acid lateral flow workflows.
What should developers optimize first?
Developers should optimize label design, amplification efficiency, probe concentration, membrane compatibility, running buffer, and background control before moving to pilot manufacturing.
Can this strip be used in POCT products?
It may be used as part of a POCT assay after full analytical validation, stability testing, usability evaluation, and regulatory review for the intended market.
Does Due Bio provide technical support?
Due Bio can support assay workflow discussion, strip selection, troubleshooting for weak lines or background, and OEM/ODM scale-up planning.