Tiger Liu What is the Hook Effect? The high-dose hook effect occurs when an excess of antigen saturates both the capture and detection antibodies, leading to a false negative or weak signal. This is a common pitfall in assays designed for high-abundance targets like CRP or Ferritin. Solution: Dilution & Membrane Capacity To mitigate this, one must … Read more
The First Line of Defense: Sample Pad Treatment In veterinary and food safety diagnostics, samples are rarely clean. Whole blood, milk, and homogenized tissue contain lipids, red blood cells, and large particulates that can clog the analytical membrane. The solution lies in the Sample Pad. Buffer Optimization for Complex Matrices Standard glass fiber pads often … Read more
Understanding Capillary Flow Time (Sec/4cm) One of the most critical parameters in Lateral Flow Assay (LFA) development is the wicking speed of the nitrocellulose membrane. Often measured as the time required for liquid to travel 4cm (e.g., HF135, HF180), choosing the wrong speed can ruin assay sensitivity. Fast vs. Slow Membranes: The Trade-off Membrane Type … Read more
The Challenge of Complex Food Matrices Detecting allergens like peanut, soy, or gluten in processed foods is notoriously difficult. Unlike clean clinical samples (serum/buffer), food extracts are often viscous, high in fats, and loaded with proteins that cause non-specific binding. This leads to the “Matrix Interference” effect, often resulting in false positives or membrane clogging. … Read more
Introduction CRISPR-Cas12a diagnostics have revolutionized nucleic acid detection, offering sensitivity comparable to PCR with the simplicity of a lateral flow readout. However, a common challenge frustrates researchers worldwide: false positives. In this guide, we explore why background signals occur in CRISPR lateral flow assays and how Due Bio’s Universal Strips help mitigate these issues through … Read more
💡 Application Note: DNA extraction-free multiplex LFA. Compatible with Due Bio’s Multiplex Universal Strips for food safety applications. Title: Meat Authenticity Made Easy: DNA Extraction-Free Rapid Onsite Detection of Duck and Pork Ingredients in Beef and Lamb Using Dual-Recombinase-Aided Amplification and Multiplex Lateral Flow Strips Source: Journal of Agricultural and Food Chemistry (ACS) (2023) Abstract … Read more
💡 Application Note: Rapid detection using RPA-CRISPR/Cas12a. This protocol validates the use of Universal Lateral Flow Strips, making it an ideal reference for Due Bio product users. Title: Development of an RPA-based CRISPR/Cas12a assay in combination with a lateral flow strip for rapid detection of toxigenic Fusarium verticillioides in maize Source: Food Control (Elsevier) (2024) … Read more
💡 Application Note: Digital CRISPR/Cas13a assay. This ultrasensitive diagnostic method is compatible with Due Bio’s RNA detection strip series. Title: Development and characterization of a digital CRISPR/Cas13a based assay for rapid and sensitive diagnosis of severe fever with thrombocytopenia syndrome virus Source: Sensors and Actuators B: Chemical (Elsevier) (2023) Abstract Severe fever with thrombocytopenia syndrome … Read more
💡 Application Note: This study establishes a universal biotinylation-based LFA platform. The methodology described is fully compatible with Due Bio’s Streptavidin-Biotin Universal Strips. Title: Biotinylation-based lateral flow assays for pathogenic and total bacteria detection Source: Analytica Chimica Acta (Elsevier) (Feb 2025) Abstract It is highly desirable to concurrently evaluate both pathogenic and total bacteria in … Read more
💡 Application Note: A critical analysis of reporter interactions in CRISPR-Cas12a LFA. The design strategies for eliminating false positives are directly applicable when using Due Bio’s optimized Lateral Flow Dipsticks. Title: Advancing Lateral Flow Detection in CRISPR/Cas12a Systems Through Rational Understanding and Design Strategies of Reporter Interactions Source: Biosensors (MDPI) (2025) Abstract CRISPR/Cas12a systems coupled … Read more