Literature

Selected Publications & Recommended Protocols

Our Universal Lateral Flow Strips and CRISPR Detection Strips (Tiosbio®) have been widely adopted by the global research community. Below is a collection of peer-reviewed articles citing our products or utilizing compatible high-performance methodologies.

Biotinylation-based Lateral Flow (2025)

Analytica Chimica Acta

A novel biotinylation-based LFA methodology compatible with Due Bio Universal Strips.

Advancing Lateral Flow in CRISPR/Cas12a (2025)

Biosensors (MDPI)

Quantifying limitations in CRISPR/Cas12a-LFT. Design strategies compatible with Due Bio strips.

Machine Learning for CRISPR LFA (2025)

Sensors & Diagnostics (RSC)

Automated interpretation of CRISPR-Cas13-based LFA using machine learning.

RPA-CRISPR/Cas12a LFS for Fusarium (2024)

Food Control (Elsevier)

Rapid detection of toxigenic Fusarium verticillioides using RPA-CRISPR/Cas12a LFS.

DNA Extraction-Free Meat Authenticity (2023)

J. Agric. Food Chem. (ACS)

Rapid onsite detection of duck and pork ingredients using dual-RAA and multiplex lateral flow strips.

Digital CRISPR/Cas13a for SFTSV (2023)

Sensors & Actuators B (Elsevier)

Development of a digital CRISPR/Cas13a based assay compatible with Due Bio detection systems.

Multiplexed CRISPR Lateral Flow (2022)

Biosensors & Bioelectronics

An orthogonal CRISPR-Cas-mediated multiplexed lateral flow assay (OC-MLFA) for SARS-CoV-2 genome detection.

Rapid Detection of Shigella spp. (2022)

PLOS ONE

Recombinase polymerase amplification combined with lateral flow dipstick (RPA-LFD) for rapid detection of Shigella and EIEC.

Bean Pod Mottle Virus (2021)

Nature Scientific Reports

A reverse transcription-cross-priming amplification method with lateral flow dipstick assay for the rapid detection of BPMV.

SARS-CoV-2 RT-RAA/LFD (2021)

Frontiers in Microbiology

Targeting the nucleocapsid (N) gene of SARS-CoV-2, specific primers and probes for RT-RAA/LFD platform.

CRISPR/Cas12a Detection of Feline Parvovirus

Frontiers in Microbiology

A diagnostic platform based on a combination of Recombinase-Aided Amplification (RAA) and CRISPR/Cas12a.

New: Read our Ultimate Guide to CRISPR-Cas12a Diagnostics for a deep dive into the technology.

Peer-Reviewed Application Matrix: Matching Literature Workflows to Due Bio SKUs
Publication / Journal Application & Target Assay Methodology Compatible Due Bio (TiosBio) Products
Analytica Chimica Acta (2025) Biotinylation-based Lateral Flow Assay Biotin/FITC Nucleic Acid LFA Universal LFA Strips (JY0201), Dual-Labeled Strips (JY0209)
Biosensors (MDPI) (2025) CRISPR/Cas12a Lateral Flow Optimization CRISPR-Cas12a Trans-Cleavage LFA Cas12/13 CRISPR Strips (JY0301), Single-System CRISPR Strips (JY0307)
Sensors & Diagnostics (RSC) (2025) Machine Learning-Assisted CRISPR LFA CRISPR-Cas13a Lateral Flow Readout Cas12/13 CRISPR Strips (JY0301)
Food Control (Elsevier) (2024) Toxigenic Fusarium verticillioides Detection RPA/RAA + CRISPR/Cas12a LFS RPApex Basic RAA Kit (JY0800) + CRISPR Strips (JY0301)
J. Agric. Food Chem. (ACS) (2023) Extraction-Free Meat Authenticity (Duck/Pork) Dual-RAA + Multiplex Lateral Flow Lightning Release Reagent (BT0066) + Dual-Target Strips (JY0208)
Biosensors & Bioelectronics (2022) Orthogonal Multiplexed SARS-CoV-2 Detection Orthogonal CRISPR-Cas Multiplex LFA Dual-System CRISPR Strips (JY0308), 3-Plex Strips (JY0230)
PLOS ONE (2022) & Nature Scientific Reports (2021) Shigella spp. & Bean Pod Mottle Virus (BPMV) RPA / RT-CPA + Lateral Flow Dipstick Universal LFA Strips (JY0201), RPApex LFS RAA Kit (JY0808)
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