Lightning Nucleic Acid Release Reagent (Universal Type) — BT0066

Tiosbio® 5× Lightning Nucleic Acid Release Reagent (Universal Type) — Cat. No. BT0066 is a rapid, enzyme-free nucleic acid release reagent for one-step sample processing before PCR, RAA, or RT-RAA amplification. It processes whole blood, serum, plasma, urine, saliva, oral swabs, nasopharyngeal swabs, and cell suspensions in 10 minutes at 95°C, without lysozyme, proteinase K, phenol, or chloroform.

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Characteristics

  1. This kit can be used for nucleic acid (both DNA and RNA) extraction, enrichment, and purification from samples including whole blood, serum, plasma, urine, saliva, oral swabs, nasopharyngeal swabs, and cell suspensions. The processed material can be directly used for PCR amplification with Tiosbio® Marathon DNA Polymerase (BT0016), and for isothermal amplification with Tiosbio® RAA Nucleic Acid Amplification Kit (JY0200), Tiosbio® RT RAA Nucleic Acid Amplification Kit (JY0203), and Tiosbio® RAA Nucleic Acid Amplification Kit (Lateral Flow Strip Method) (JY0202).
  2. No addition of enzymatic preparations such as lysozyme, lysostaphin, or proteinase K is required. No use of harmful chemical reagents such as phenol or chloroform is needed.
  3. One-step, single-tube operation that is convenient and fast, with an operation time of only 10 minutes.
  4. High sensitivity and good reproducibility. After processing with this kit, taking 1 μL of lysate supernatant as template for PCR can stably amplify target genes with an input of 10¹ copies.
  5. Can be used for sample pretreatment in food safety, disease control, emerging infectious diseases, animal epidemics, molecular diagnostics, and genetic testing for precision medicine.

Specifications and Storage

ItemDetails
Catalog NumberBT0066
Product NameTiosbio® 5× Lightning Nucleic Acid Release Reagent (Universal Type)
Specifications500 μL per tube
Storage Conditions-20°C
Shelf Life24 months
Operation Time10 minutes
Sample-to-Reagent Ratio4:1

Procedure

After processing samples such as whole blood, serum, plasma, urine, saliva, oral swabs, nasopharyngeal swabs, and cell suspensions, mix and process with Tiosbio® 5× Lightning Nucleic Acid Release Reagent (BT0066) at a ratio of 4:1.

Recommended System and Usage Method

  1. For every 20 μL of sample, add 5 μL of 5× Lightning Nucleic Acid Release Reagent (BT0066) to a new 200 μL Eppendorf tube.
  2. After vortex mixing thoroughly, place the Eppendorf tube on a metal bath or thermal cycler and heat at 95°C for 10 minutes.
  3. Remove the Eppendorf tube and equilibrate to room temperature.
  4. Centrifuge at 12,000 rpm for 2 minutes.
  5. Aspirate the supernatant from the Eppendorf tube into a clean centrifuge tube for testing.
  6. For PCR, RAA, or RT RAA amplification, add 1 μL of processed supernatant per 25 μL reaction system.

Warnings and Precautions

  1. When using this kit for nucleic acid extraction from whole blood, serum, plasma, urine, saliva, oral swabs, nasopharyngeal swabs, or cell suspensions, sample processing should be completed immediately after sample collection whenever possible. If conditions do not allow, samples should be transported under refrigerated or frozen conditions.
  2. When used for infectious disease sample testing, pay attention to safety protection during operation. Operations should be conducted in designated laboratory facilities. Wear protective clothing, disposable gloves, and masks. All items that have directly contacted pathogen samples should be disinfected before disposal or reuse.
  3. This kit is designed for rapid crude processing of animal liquid samples. The processed material can be used for PCR amplification with Tiosbio® Marathon DNA Polymerase (BT0016), and for isothermal amplification with Tiosbio® RAA Nucleic Acid Amplification Kit (JY0200), Tiosbio® RT RAA Nucleic Acid Amplification Kit (JY0203), and Tiosbio® RAA Nucleic Acid Amplification Kit (Lateral Flow Strip Method) (JY0202). The processed material is not suitable for experiments requiring high nucleic acid purity, such as UV spectrophotometric quantification.
  4. When using the 5× Lightning Nucleic Acid Release Reagent, the ratio of sample to reagent should be 4:1. This ratio has been repeatedly optimized. Do not adjust arbitrarily!
  5. For PCR, RAA, or RT RAA amplification, only 1 μL of processed supernatant is needed per 25 μL reaction system! Do not arbitrarily increase or decrease the amount of processed supernatant added. When the amplification template is DNA, the stored processed supernatant at -20°C can be reused multiple times within 48 hours.
  6. The success of PCR or RAA reactions is closely related to primer design, cycling parameters, and amplification time settings. If amplification fails after processing samples with this kit, please confirm whether PCR or RAA reactions were successful using nucleic acid extracted by other methods.
  7. This reagent is for research use only. Do not use for clinical, food, or other purposes.

Compatible Downstream Amplification Products

  • PCR: Tiosbio® Marathon DNA Polymerase (BT0016)
  • RAA Isothermal Amplification: Tiosbio® RAA Nucleic Acid Amplification Kit (JY0200)
  • RT-RAA Isothermal Amplification: Tiosbio® RT RAA Nucleic Acid Amplification Kit (JY0203)
  • RAA + Lateral Flow Readout: Tiosbio® RAA Nucleic Acid Amplification Kit — Lateral Flow Strip Method (JY0202)

FAQ: Lightning Nucleic Acid Release Reagent (BT0066)

What is the Lightning Nucleic Acid Release Reagent used for?

BT0066 is a universal one-step nucleic acid release reagent for extracting DNA and RNA from whole blood, serum, plasma, urine, saliva, oral swabs, nasopharyngeal swabs, and cell suspensions. The processed lysate can be directly used as template for PCR, RAA, or RT-RAA amplification without further purification.

Is this reagent enzyme-free?

Yes. BT0066 does not require lysozyme, lysostaphin, or proteinase K, and does not use phenol or chloroform. This simplifies the workflow and avoids hazardous organic solvents typical in traditional extraction protocols.

How long does one sample take to process?

10 minutes at 95°C, plus 2 minutes centrifugation. Total hands-on time is around 12–15 minutes per batch, which makes BT0066 suitable for point-of-care molecular workflows and rapid isothermal amplification pipelines.

What sensitivity can be achieved with BT0066 as input?

With 1 μL of lysate supernatant as template, PCR can stably amplify targets from an input of 10¹ copies, based on Tiosbio internal validation data.

Which downstream assays are supported?

PCR (Marathon DNA Polymerase BT0016), RAA (JY0200), RT-RAA (JY0203), and RAA + lateral flow strip readout (JY0202). BT0066 is optimized as a sample prep front-end for Tiosbio isothermal amplification and CRISPR lateral flow workflows.

Is BT0066 suitable for high-purity applications like UV quantification?

No. BT0066 is a rapid crude release reagent. The processed material is not suitable for experiments requiring high nucleic acid purity such as UV spectrophotometric quantification.

Is BT0066 registered as an IVD reagent?

This reagent is supplied for research use only. IVD registration status depends on the buyer’s target market and legal manufacturer responsibilities. Distributors and OEM partners planning IVD registration should contact Due Bio to discuss documentation, technical file support, and private-label options.

Does Due Bio support OEM/ODM for BT0066?

Yes. Due Bio / TiosBio supports OEM packaging, private-label formulations, bulk supply, and customized documentation for qualified distributors and diagnostic manufacturers. Contact Due Bio with your target market, expected annual volume, and packaging requirements to receive a distributor evaluation quote.

Related Technical Guides

Tiosbio® 5× Lightning Nucleic Acid Release Reagent (BT0066) is supplied for research use only. For OEM/ODM cooperation, distributor quotations, or regulatory documentation requests, contact Due Bio through the Contact page.