LF-detect: Dual-system dual-target CRISPR Lateral flow strip

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Cat. No. JY0308

Product Overview

LF-detect Dual-system dual-target CRISPR Lateral flow strip for simultaneous detection of Cas12 and Cas13 enzyme cleavage products.

Working Principle

This product uses lateral flow dual-antibody sandwich assay to detect Cas12 and Cas13 enzyme cleavage products. After the target gene is amplified by LAMP, RAA/RPA, or PCR, Cas12 and Cas13 enzymes are used to simultaneously cleave the amplification products.

The DNA signal probe A corresponding to Cas12 enzyme and the RNA signal probe B corresponding to Cas13 enzyme are modified as follows:

  1. Probe A: Biotin at one end and FITC or 6-FAM at the other end; Probe B: Biotin at one end and Digoxin (Dig) at the other end
  2. Or: Probe A: Biotin at one end and Digoxin (Dig) at the other end; Probe B: Biotin at one end and FITC or 6-FAM at the other end

Intended Use

Detection of products after Cas12 and Cas13 dual-enzyme cleavage.

Package Specification

  • 50 strips/tube, aluminum foil bag moisture-proof packaging

Storage Conditions and Shelf Life

  • Storage: Store in a dark and dry place at 4-30°C
  • Shelf Life: 12 months

Procedure

  1. Take out the corresponding number of test strips according to the number of samples to be tested, and mark them on the absorbent pad. Each test strip can only be used for single detection of products from simultaneous cleavage of DNA probe and RNA probe. When the enzyme cleavage product volume is 50-100 μL, the nucleic acid product can be directly detected in a 200 μL PCR reaction tube. When the product volume is less than 50 μL, ultrapure water needs to be added to make up the volume to 50 μL, mix thoroughly by pipetting, and then detection can be performed.
  2. After the CRISPR system enzyme cleavage reaction is completed, open the PCR reaction tube and insert the conjugate pad end (arrow end) of the test strip into the PCR reaction tube. The liquid level must not exceed the top of the conjugate pad. Wait for the reading zone to be fully wetted (approximately 1-2 minutes). After the test line (T line) develops color, the test strip can be removed.
  3. Observe the results within 10 minutes after the control line (C line) develops color. Reading after 10 minutes is invalid.
  4. Record the detection results and seal and discard the test strips in a safe place.

Download

📄 Download IFU (JY0308_EN.pdf)

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