Pushing Detection Limits in Time-Resolved Fluorescence Immunoassays
Time-resolved fluorescence (TRF) assays using europium chelates offer superior sensitivity compared to conventional fluorescence, but practical detection limits depend on multiple factors beyond the fluorophore itself. This article examines real-world sensitivity barriers and optimization strategies. Theoretical vs. Practical Sensitivity Europium chelate TRF specifications: Theoretical limit: 0.01 pg/mL (based on photon emission rates) Practical limit in … Read more