Selected Publications & Recommended Protocols
Our Universal Lateral Flow Strips and CRISPR Detection Strips (Tiosbio®) have been widely adopted by the global research community. Below is a collection of peer-reviewed articles citing our products or utilizing compatible high-performance methodologies.

Biotinylation-based Lateral Flow (2025)
Analytica Chimica Acta
A novel biotinylation-based LFA methodology compatible with Due Bio Universal Strips.

Advancing Lateral Flow in CRISPR/Cas12a (2025)
Biosensors (MDPI)
Quantifying limitations in CRISPR/Cas12a-LFT. Design strategies compatible with Due Bio strips.

Machine Learning for CRISPR LFA (2025)
Sensors & Diagnostics (RSC)
Automated interpretation of CRISPR-Cas13-based LFA using machine learning.

RPA-CRISPR/Cas12a LFS for Fusarium (2024)
Food Control (Elsevier)
Rapid detection of toxigenic Fusarium verticillioides using RPA-CRISPR/Cas12a LFS.

DNA Extraction-Free Meat Authenticity (2023)
J. Agric. Food Chem. (ACS)
Rapid onsite detection of duck and pork ingredients using dual-RAA and multiplex lateral flow strips.

Digital CRISPR/Cas13a for SFTSV (2023)
Sensors & Actuators B (Elsevier)
Development of a digital CRISPR/Cas13a based assay compatible with Due Bio detection systems.

Multiplexed CRISPR Lateral Flow (2022)
Biosensors & Bioelectronics
An orthogonal CRISPR-Cas-mediated multiplexed lateral flow assay (OC-MLFA) for SARS-CoV-2 genome detection.

Rapid Detection of Shigella spp. (2022)
PLOS ONE
Recombinase polymerase amplification combined with lateral flow dipstick (RPA-LFD) for rapid detection of Shigella and EIEC.

Bean Pod Mottle Virus (2021)
Nature Scientific Reports
A reverse transcription-cross-priming amplification method with lateral flow dipstick assay for the rapid detection of BPMV.

SARS-CoV-2 RT-RAA/LFD (2021)
Frontiers in Microbiology
Targeting the nucleocapsid (N) gene of SARS-CoV-2, specific primers and probes for RT-RAA/LFD platform.

CRISPR/Cas12a Detection of Feline Parvovirus
Frontiers in Microbiology
A diagnostic platform based on a combination of Recombinase-Aided Amplification (RAA) and CRISPR/Cas12a.
New: Read our Ultimate Guide to CRISPR-Cas12a Diagnostics for a deep dive into the technology.
Peer-Reviewed Application Matrix: Matching Literature Workflows to Due Bio SKUs| Publication / Journal | Application & Target | Assay Methodology | Compatible Due Bio (TiosBio) Products |
|---|---|---|---|
| Analytica Chimica Acta (2025) | Biotinylation-based Lateral Flow Assay | Biotin/FITC Nucleic Acid LFA | Universal LFA Strips (JY0201), Dual-Labeled Strips (JY0209) |
| Biosensors (MDPI) (2025) | CRISPR/Cas12a Lateral Flow Optimization | CRISPR-Cas12a Trans-Cleavage LFA | Cas12/13 CRISPR Strips (JY0301), Single-System CRISPR Strips (JY0307) |
| Sensors & Diagnostics (RSC) (2025) | Machine Learning-Assisted CRISPR LFA | CRISPR-Cas13a Lateral Flow Readout | Cas12/13 CRISPR Strips (JY0301) |
| Food Control (Elsevier) (2024) | Toxigenic Fusarium verticillioides Detection | RPA/RAA + CRISPR/Cas12a LFS | RPApex Basic RAA Kit (JY0800) + CRISPR Strips (JY0301) |
| J. Agric. Food Chem. (ACS) (2023) | Extraction-Free Meat Authenticity (Duck/Pork) | Dual-RAA + Multiplex Lateral Flow | Lightning Release Reagent (BT0066) + Dual-Target Strips (JY0208) |
| Biosensors & Bioelectronics (2022) | Orthogonal Multiplexed SARS-CoV-2 Detection | Orthogonal CRISPR-Cas Multiplex LFA | Dual-System CRISPR Strips (JY0308), 3-Plex Strips (JY0230) |
| PLOS ONE (2022) & Nature Scientific Reports (2021) | Shigella spp. & Bean Pod Mottle Virus (BPMV) | RPA / RT-CPA + Lateral Flow Dipstick | Universal LFA Strips (JY0201), RPApex LFS RAA Kit (JY0808) |