Mitigating the Hook Effect in High-Viral Load Samples

False Negatives at Peak Infection

In acute phases (e.g., COVID-19 or CRP spikes), excess antigen can saturate antibodies, causing the Test line to fade. This “Hook Effect” is dangerous.

Three-Line Architecture

We recommend adding a third “Hook Line” or using Due Bio’s high-capacity sample pads to sequester excess antigen, extending the linear range of your assay.