Star Flash Nucleic Acid Release Reagent — Cat. No. BT0069 is a sub-minute enzyme-free nucleic acid release reagent optimized for RAA/RPA isothermal amplification.
Characteristics
- This reagent can be used for nucleic acid (both DNA and RNA) extraction, enrichment, and purification from
- animal samples including saliva, oral swabs, nasopharyngeal swabs, cell suspensions, and tissue blocks, or plant
- samples including roots, stems, leaves, flowers, and seeds. The product after lysate treatment can be directly used for
- isothermal amplification with Tiosbio® RAA Nucleic Acid Amplification Kit (JY0200), Tiosbio® RT RAA Nucleic
- Acid Amplification Kit (JY0203), and Tiosbio® RAA Nucleic Acid Amplification Kit (Lateral Flow Strip Method)
- No addition of enzymatic preparations such as lysozyme, lysostaphin, or proteinase K is required. No use of
Specifications and Storage
| Item | Details |
|---|---|
| Catalog Number | BT0069 |
| Product Name | Star Flash Nucleic Acid Release Reagent |
| Operation Time | < 1 minute |
| Storage Conditions | Room temperature |
| Shelf Life | 12 months |
Procedure
1. Animal Samples After appropriate processing of samples such as saliva, oral swabs, nasopharyngeal swabs, and cell suspensions, mix with the sample to be tested at a ratio of Star Flash Nucleic Acid Release Reagent (BT0069) to liquid sample = 1:4. For tissue samples, lyse at a ratio of 200 μL lysis solution per 100-200 mg of sample. Crush the material to be tested with a pipette tip for thorough grinding and fragmentation of the sample, then blow and aspirate the lysis solution several times with a pipette tip or disposable plastic pipette, or vortex to mix the solution. Blow and aspirate the lysis solution several times with a pipette tip or disposable plastic pipette, or vortex to mix the solution, centrifuge briefly, and aspirate the supernatant into a clean centrifuge tube for testing. For RAA or RT RAA amplification, the lysate supernatant can be added up to the maximum allowable volume of the reaction system. 2. Plant Samples Cut the material to be tested into 5 mm square small pieces or segments, add 50 μL of Star Flash Nucleic Acid Release Reagent (BT0069), and crush the material with a pipette tip. After thorough grinding and fragmentation of the sample, blow and aspirate the lysis solution several times with a pipette tip or disposable plastic pipette, or vortex to mix the solution. After brief centrifugation of the lysis solution, aspirate the supernatant into a clean centrifuge tube for testing. For RAA or RT RAA amplification, 2 μL of lysate supernatant can be taken for detection. Addition amount of lysate in amplification system: The lysis solution will inhibit enzyme activity. If the detection results of the sample to be tested, positive control, and negative control samples after lysis do not match the actual situation, parallel tests can be set up with a gradient of ±2 μL of lysate addition volume to determine the effect of different lysate volumes on the detection of samples, positive controls, and negative controls within the same amplification system. Under the condition that the positive control detection result is positive and the negative control detection result is negative, select the maximum lysate addition amount. Since Star Flash Nucleic Acid Release Reagent has the effect of inhibiting non-specific amplification, when all negative controls are detected as negative and all positive controls are detected as positive, the lysate can be added at the maximum sample addition volume allowed by the amplification system.
Warnings and Precautions
- When using this reagent for nucleic acid extraction from saliva, oral swabs, nasopharyngeal swabs, or cell
- suspensions, sample processing and amplification should be completed immediately after sample collection whenever
- possible. If conditions do not allow, samples should be transported under refrigerated or frozen conditions.
- When used for infectious disease sample testing, pay attention to safety protection during operation.
- Operations should be conducted in designated laboratory facilities. Wear protective clothing, disposable gloves, and
- masks. All items that have directly contacted pathogen samples should be disinfected before disposal or reuse.
- This reagent is designed for rapid crude processing of animal liquid samples. The processed material can be
- used for isothermal amplification with Tiosbio® RAA Nucleic Acid Amplification Kit (JY0200), Tiosbio® RT RAA
- Nucleic Acid Amplification Kit (JY0203), and Tiosbio® RAA Nucleic Acid Amplification Kit (Lateral Flow Strip
- Method) (JY0202). The processed material is not suitable for experiments requiring high nucleic acid purity, such as
FAQ: Star Flash Nucleic Acid Release Reagent
What is BT0069 used for?
BT0069 is a sub-minute enzyme-free nucleic acid release reagent optimized for RAA/RPA isothermal amplification. It is designed for rapid sample processing in molecular diagnostics and research applications.
Does Due Bio support OEM/ODM for BT0069?
Yes. Due Bio / TiosBio supports OEM packaging, private-label formulations, bulk supply, and customized documentation for qualified distributors and diagnostic manufacturers. Contact Due Bio with your target market, expected annual volume, and packaging requirements.
Related Technical Guides
This product is supplied for research use only. For OEM/ODM cooperation, distributor quotations, or regulatory documentation requests, contact Due Bio through the Contact page.