This product uses a chromatographic double-antibody sandwich method to detect the enzyme cleavage products of Cas12 and Cas13. After the target gene is amplified by LAMP, RAA/RPA, and PCR, use Cas12 and Cas13 enzymes to cut the amplified product at the same time, and the DNA signal probe A corresponding to the Cas12 enzyme (Cas12 cleavage), or the RNA signal probe corresponding to the Cas13 enzyme Needle B (Cas13 cleavage) is modified as follows, then this product can be used for detection of cleavage products:
- One end of probe A is modified with biotin (Biotin), the other end is modified with fluorescein isothiocyanate (FITC) or 6-carboxyfluorescein (6-FAM), and one end of probe B is modified with biotin (Biotin) , the other end is modified with digoxin (Dig);
- Or, one end of probe A is modified with biotin (Biotin), the other end is modified with digoxin (Dig), and one end of probe B is modified with biotin (Biotin), and the other end is modified with fluorescein isothiocyanate ( FITC) or 6-carboxyfluorescein (6-FAM).
FAQ: CRISPR Double Enzyme Cutting Nucleic Acid Detection Strip
What is a CRISPR double enzyme cutting detection strip?
It is a lateral flow strip format designed for CRISPR workflows where dual enzyme or dual-system signal strategies may be used to detect nucleic acid targets with strip-based readout.
Is this product suitable for multiplex CRISPR detection?
It can be evaluated for advanced CRISPR assay designs, including dual-target or dual-system workflows, but multiplex performance depends on reporter design, capture chemistry, and assay validation.
What problems should developers watch for?
Common problems include false background, weak test lines, reporter imbalance, nonspecific cleavage, amplification contamination, and inconsistent flow caused by sample matrix or buffer conditions.
Who are typical buyers?
Typical buyers include IVD R&D teams, molecular diagnostics manufacturers, CRISPR assay developers, reference labs, and distributors building a molecular POCT portfolio.
Can Due Bio help with customization?
Yes. Due Bio can discuss customized strip formats, pilot batches, private-label supply, and technical troubleshooting for CRISPR lateral flow assay development.