Troubleshooting

Troubleshooting Invalid Tests: Why Does the Control Line Fail?

by

No C-Line = No Result A missing Control (C) line renders the test invalid. Common causes include: Conjugate Death: Gold nanoparticles aggregated or antibodies denatured. Membrane Dry-out: Old strips stored without desiccants. Flow Obstruction: Sample pad clogging preventing liquid from reaching the C-line. Switching to Due Bio robustly packaged and stabilized strips can reduce invalid … Read more

Troubleshooting Guide: Solving False Positives in CRISPR-Cas12a Lateral Flow Assays

by

Introduction CRISPR-Cas12a diagnostics have revolutionized nucleic acid detection, offering sensitivity comparable to PCR with the simplicity of a lateral flow readout. However, a common challenge frustrates researchers worldwide: false positives. In this guide, we explore why background signals occur in CRISPR lateral flow assays and how Due Bio’s Universal Strips help mitigate these issues through … Read more