The 40nm Standard For most lateral flow assays, 40nm gold is the industry standard. It offers the best balance between color intensity (optical density) and steric stability against aggregation. When to Use 20nm? Smaller 20nm particles have a higher surface-area-to-volume ratio, allowing for higher antibody loading density. This can be advantageous for competitive assays where … Read more
Why Fluorescence? Europium nanobeads offer 100x higher sensitivity than colloidal gold due to Time-Resolved Fluorescence (TRF) which eliminates background noise. Membrane Autofluorescence Standard nitrocellulose glows under UV light, killing your signal. Due Bio offers a special “Low-Fluorescence” grade membrane specifically cast for TRF readers.
More Than Just Saline A running buffer isn’t just a vehicle; it’s a chemical tool. To boost sensitivity in Due Bio Universal Strips, we recommend tuning your buffer with: Surfactants (0.5-2%): Tween-20 or Triton X-100 to reduce hydrophobic interactions. Blocking Proteins (1% BSA): To coat the membrane surface and prevent background. Viscosity Modifiers (PVP/PEG): To … Read more
More Than Just Saline A running buffer isn’t just a vehicle; it’s a chemical tool. To boost sensitivity in Due Bio Universal Strips, we recommend tuning your buffer with: Surfactants (0.5-2%): Tween-20 or Triton X-100 to reduce hydrophobic interactions. Blocking Proteins (1% BSA): To coat the membrane surface and prevent background. Viscosity Modifiers (PVP/PEG): To … Read more
The ASFV Challenge African Swine Fever Virus (ASFV) requires early detection to prevent herd devastation. Standard colloidal gold strips often miss low viral loads in the incubation phase. Enhancing Sensitivity To detect ASFV at pogram levels, we recommend using Due Bio High-Bind Nitrocellulose combined with Europium bead labels (fluorescent LFA). Our membranes are optimized for … Read more
Flow Rate vs. Surface Area Smaller pores (e.g., 8μm) offer higher surface area for antibody binding and slower flow, increasing sensitivity. Larger pores (12-15μm) allow faster flow but reduce reaction time. Selection Strategy For high-sensitivity CRISPR assays, we typically recommend 10μm or 12μm membranes as the optimal balance. Due Bio provides a range of defined … Read more
Understanding Capillary Flow Time (Sec/4cm) One of the most critical parameters in Lateral Flow Assay (LFA) development is the wicking speed of the nitrocellulose membrane. Often measured as the time required for liquid to travel 4cm (e.g., HF135, HF180), choosing the wrong speed can ruin assay sensitivity. Fast vs. Slow Membranes: The Trade-off Membrane Type … Read more