Small Molecule Detection: Optimizing Competitive Assays for Pesticides
Competitive vs. Sandwich Unlike COVID-19 (sandwich assay), detecting small molecules like Glyphosate or Antibiotics requires a Competitive Assay format. Here, the T-line intensity decreases as analyte concentration increases. Critical Tuning Competitive assays are highly sensitive to membrane flow rate. A slower wicking speed (e.g., 180s/4cm) is crucial to allow sufficient competition at the test line. … Read more