Flow Rate vs. Surface Area Smaller pores (e.g., 8μm) offer higher surface area for antibody binding and slower flow, increasing sensitivity. Larger pores (12-15μm) allow faster flow but reduce reaction time. Selection Strategy For high-sensitivity CRISPR assays, we typically recommend 10μm or 12μm membranes as the optimal balance. Due Bio provides a range of defined … Read more
Understanding Capillary Flow Time (Sec/4cm) One of the most critical parameters in Lateral Flow Assay (LFA) development is the wicking speed of the nitrocellulose membrane. Often measured as the time required for liquid to travel 4cm (e.g., HF135, HF180), choosing the wrong speed can ruin assay sensitivity. Fast vs. Slow Membranes: The Trade-off Membrane Type … Read more
The Challenge of Complex Food Matrices Detecting allergens like peanut, soy, or gluten in processed foods is notoriously difficult. Unlike clean clinical samples (serum/buffer), food extracts are often viscous, high in fats, and loaded with proteins that cause non-specific binding. This leads to the “Matrix Interference” effect, often resulting in false positives or membrane clogging. … Read more