Multiplexing

Design Rules for Multiplex Lateral Flow Assays (2+ Lines)

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The Spacing Rule To prevent optical cross-talk or “carry-over” between Test Line 1 and Test Line 2, we recommend a minimum spacing of 3mm. This allows the reading window to resolve distinct bands. Flow Dynamics In multiplex assays, the downstream line (T2) always sees less analyte than the upstream line (T1). Due Bio can provide … Read more

The Future of Multiplexing in Lateral Flow

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Detecting Multiple Targets Multiplexing allows for the simultaneous detection of Flu A, Flu B, and COVID-19 on a single strip. This requires precise spacing of test lines. Manufacturing Precision Due Bio offers Custom Strip Cutting services with high-precision line dispensing compatibility, ensuring distinct separation even for closely spaced targets.