LFA Troubleshooting

Eliminating Ghost Lines: Causes and Fixes

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The Mystery of Ghost Lines Ghost lines are faint, non-specific signals that appear at the test line location even in negative samples. They are often caused by antibody aggregation or insufficient blocking. Optimization Strategy Ensure your conjugate pad is pre-treated with a robust blocking buffer. Due Bio strips come with a proprietary Low-Noise Pre-treatment that … Read more

Troubleshooting the High-Dose Hook Effect in Lateral Flow Assays

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What is the Hook Effect? The high-dose hook effect occurs when an excess of antigen saturates both the capture and detection antibodies, leading to a false negative or weak signal. This is a common pitfall in assays designed for high-abundance targets like CRP or Ferritin. Solution: Dilution & Membrane Capacity To mitigate this, one must … Read more