Introduction: The CRISPR Diagnostics Revolution Since the discovery of Cas12a (Cpf1) collateral cleavage activity, molecular diagnostics has shifted from lab-bound PCR to field-deployable DETECTR and HOLMES assays. This comprehensive guide covers everything you need to know about setting up a high-sensitivity CRISPR-Cas12a lateral flow system. Chapter 1: Mechanism of Action Unlike Cas9, which targets DNA … Read more
The “Walking Pneumonia” Surge With the recent outbreaks of Mycoplasma pneumoniae in Asia and Europe, demand for rapid IgM/IgG detection is soaring. Sensitivity is key for pediatric samples. High-Flow Membranes Due Bio recommends using our HF135 Universal Strips. The balanced flow rate ensures sufficient antigen capture time for the relatively large Mycoplasma bacteria, boosting clinical … Read more
The Challenge of Complex Food Matrices Detecting allergens like peanut, soy, or gluten in processed foods is notoriously difficult. Unlike clean clinical samples (serum/buffer), food extracts are often viscous, high in fats, and loaded with proteins that cause non-specific binding. This leads to the “Matrix Interference” effect, often resulting in false positives or membrane clogging. … Read more
Introduction CRISPR-Cas12a diagnostics have revolutionized nucleic acid detection, offering sensitivity comparable to PCR with the simplicity of a lateral flow readout. However, a common challenge frustrates researchers worldwide: false positives. In this guide, we explore why background signals occur in CRISPR lateral flow assays and how Due Bio’s Universal Strips help mitigate these issues through … Read more