Antibody Pairing Strategy for Sandwich Lateral Flow Assays
Capture vs. Detection In a sandwich assay, the Capture Antibody (on the T-line) and Detection Antibody (on the Gold) must bind to different epitopes. Steric hindrance is a common failure mode. Screening Protocol We recommend a “Checkerboard” screening approach using Due Bio Universal Strips. Test Antibody A (Capture) against Antibody B (Detection), and then swap … Read more