Passive Adsorption vs. Covalent Binding in Gold Conjugation
Passive Adsorption The traditional method relies on electrostatic and hydrophobic interactions. It is simple and cost-effective but can be unstable with certain antibodies. Covalent Binding (EDC/NHS) For higher stability and reproducibility, covalent coupling using EDC/NHS chemistry is superior. Due Bio recommends optimized conjugation buffers to maximize antibody orientation on the gold nanoparticle surface.