Introduction: The CRISPR Diagnostics Revolution Since the discovery of Cas12a (Cpf1) collateral cleavage activity, molecular diagnostics has shifted from lab-bound PCR to field-deployable DETECTR and HOLMES assays. This comprehensive guide covers everything you need to know about setting up a high-sensitivity CRISPR-Cas12a lateral flow system. Chapter 1: Mechanism of Action Unlike Cas9, which targets DNA … Read more
The Invisible Enemy In CRISPR-Cas12/13 diagnostics, RNA reporters are susceptible to rapid degradation by ubiquitous RNAses, leading to false positives (premature cleavage). Buffer Additives Incorporating RNAse inhibitors is standard. However, the lateral flow strip itself must be RNAse-free. Due Bio manufacturing lines are ISO-certified and strictly controlled for nuclease contamination.
Introduction CRISPR-Cas12a diagnostics have revolutionized nucleic acid detection, offering sensitivity comparable to PCR with the simplicity of a lateral flow readout. However, a common challenge frustrates researchers worldwide: false positives. In this guide, we explore why background signals occur in CRISPR lateral flow assays and how Due Bio’s Universal Strips help mitigate these issues through … Read more