Optimizing Your Running Buffer: The Secret Ingredient for Sensitivity

More Than Just Saline

A running buffer isn’t just a vehicle; it’s a chemical tool. To boost sensitivity in Due Bio Universal Strips, we recommend tuning your buffer with:

  • Surfactants (0.5-2%): Tween-20 or Triton X-100 to reduce hydrophobic interactions.
  • Blocking Proteins (1% BSA): To coat the membrane surface and prevent background.
  • Viscosity Modifiers (PVP/PEG): To slow down flow rate and increase reaction time.

Leave a Comment