The First Line of Defense: Sample Pad Treatment
In veterinary and food safety diagnostics, samples are rarely clean. Whole blood, milk, and homogenized tissue contain lipids, red blood cells, and large particulates that can clog the analytical membrane. The solution lies in the Sample Pad.
Buffer Optimization for Complex Matrices
Standard glass fiber pads often fail to filter these contaminants. At Due Bio, we treat our Universal Strips with a proprietary buffer containing:
- Blocking Agents (BSA/Casein): To prevent non-specific protein adsorption.
- Surfactants (Tween/Triton): To lyse residual membranes and aid flow.
- pH Stabilizers: To ensure the conjugate releases at the optimal pH.
Impact on Signal Clarity
Proper pre-treatment can increase the Signal-to-Noise (S/N) ratio by up to 40%. This is why Due Bio’s strips are preferred for African Swine Fever (ASFV) and Food Allergen testing, where sample matrices are notoriously difficult.
Reference: Methods in Molecular Biology (2025), Chapter on LFA Optimization.