Publications

💡 Application Note: DNA extraction-free multiplex LFA. Compatible with Due Bio’s Multiplex Universal Strips for food safety applications. Title: Meat Authenticity Made Easy: DNA Extraction-Free Rapid Onsite Detection of Duck and Pork Ingredients in Beef and Lamb Using Dual-Recombinase-Aided Amplification and Multiplex Lateral Flow Strips Source: Journal of Agricultural and Food Chemistry (ACS) (2023) Abstract … Read more

💡 Application Note: Rapid detection using RPA-CRISPR/Cas12a. This protocol validates the use of Universal Lateral Flow Strips, making it an ideal reference for Due Bio product users. Title: Development of an RPA-based CRISPR/Cas12a assay in combination with a lateral flow strip for rapid detection of toxigenic Fusarium verticillioides in maize Source: Food Control (Elsevier) (2024) … Read more

💡 Application Note: Digital CRISPR/Cas13a assay. This ultrasensitive diagnostic method is compatible with Due Bio’s RNA detection strip series. Title: Development and characterization of a digital CRISPR/Cas13a based assay for rapid and sensitive diagnosis of severe fever with thrombocytopenia syndrome virus Source: Sensors and Actuators B: Chemical (Elsevier) (2023) Abstract Severe fever with thrombocytopenia syndrome … Read more

💡 Application Note: This study establishes a universal biotinylation-based LFA platform. The methodology described is fully compatible with Due Bio’s Streptavidin-Biotin Universal Strips. Title: Biotinylation-based lateral flow assays for pathogenic and total bacteria detection Source: Analytica Chimica Acta (Elsevier) (Feb 2025) Abstract It is highly desirable to concurrently evaluate both pathogenic and total bacteria in … Read more

💡 Application Note: A critical analysis of reporter interactions in CRISPR-Cas12a LFA. The design strategies for eliminating false positives are directly applicable when using Due Bio’s optimized Lateral Flow Dipsticks. Title: Advancing Lateral Flow Detection in CRISPR/Cas12a Systems Through Rational Understanding and Design Strategies of Reporter Interactions Source: Biosensors (MDPI) (2025) Abstract CRISPR/Cas12a systems coupled … Read more

💡 Tech Spotlight: Machine Learning for LFA interpretation. Due Bio’s high-consistency manufacturing ensures the strip-to-strip uniformity required for accurate AI-based diagnostics. Title: Rapid and automated interpretation of CRISPR-Cas13-based lateral flow assay test results using machine learning Source: Sensors & Diagnostics (RSC) (2025) Abstract CRISPR-Cas-based lateral flow assays (LFAs) have emerged as a promising diagnostic tool … Read more

Title: Multiplexed lateral flow assay integrated with orthogonal CRISPR-Cas system for SARS-CoV-2 detection Source: Biosensors and Bioelectronics (PMC9396441) Abstract The development of field-deployable detection platform amenable for multiplexed genes testing will significantly improve the efficiency and reliability during point-of-care testing (POCT) applications. In this regard, an orthogonal CRISPR-Cas-mediated multiplexed lateral flow assay (designated as OC-MLFA) … Read more

Title: A reverse transcription-cross-priming amplification method with lateral flow dipstick assay for the rapid detection of Bean pod mottle virus Source: Scientific Reports (Nature) Introduction Bean pod mottle virus (BPMV) is a comovirus that has a bipartite positive-strand RNA genome consisting of RNA-1 and RNA-2. It has become one of the most economically important soybean … Read more

Title: Reverse Transcription Recombinase-Aided Amplification Assay With Lateral Flow Dipstick Assay for Rapid Detection of 2019 Novel Coronavirus Source: Frontiers in Cellular and Infection Microbiology (PMC7882697) Abstract Background: The emerging Coronavirus Disease-2019 (COVID-19) has challenged the public health globally. With the increasing requirement of detection for SARS-CoV-2 outside of the laboratory setting, a rapid and … Read more

Title: Rapid detection of feline parvovirus using RAA-CRISPR/Cas12a-based lateral flow strip and fluorescence Source: Frontiers in Microbiology (PMC11932995) Abstract Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats, with high morbidity and mortality, necessitating a rapid and effective antigen diagnostic test with high sensitivity and specificity. In this study, a diagnostic platform based on … Read more