💡 Application Note: This study establishes a universal biotinylation-based LFA platform. The methodology described is fully compatible with Due Bio’s Streptavidin-Biotin Universal Strips.
Title: Biotinylation-based lateral flow assays for pathogenic and total bacteria detection
Source: Analytica Chimica Acta (Elsevier) (Feb 2025)
Abstract
It is highly desirable to concurrently evaluate both pathogenic and total bacteria in water and food environments. As a point-of-care platform for biochemical tests, lateral flow assay (LFA) has been widely used for pathogenic bacteria due to its portability and fast time of outcome. However, traditional LFA was unable to detect total bacteria due to the lack of a universal antibody that could bind all the bacteria. Here, we used sulfo–NHS–biotin to label nonspecifically the lipoprotein of bacterial surface. Thus, a biotinylation-based LFA was established for total bacteria detection. Taking the advantage of the universal recognition reaction between the biotin and streptavidin, we established biotinylation-based LFAs for both pathogenic and total bacteria detection without the de novo preparation of the test strips.