Cat. No. JY0800
Product Overview
RPApex Basic uses enhanced Recombinase Aided Amplification (RAA) technology for rapid isothermal amplification at 39–42℃. Ready-to-use lyophilized pellets for easy transport and storage.
Principle
- Recombinase-primer complex invades target DNA template
- Single-strand binding proteins maintain open-chain state
- DNA polymerase initiates strand synthesis at 3′ end
- Exponential amplification achieved in 20–40 minutes
Intended Use
- For research use only (RUO)
- Amplification of DNA and RNA templates
- Applications: CRISPR detection, agarose gel electrophoresis analysis
Materials Supplied
- 48 reactions per kit
- Lyophilized pellets containing: recombinase, DNA polymerase, SSB, ATP, dNTPs, buffer components
- Note: Mg²⁺ not included by default. Recommended final concentration: 14 mM/Test. Specify when ordering if required.
Storage and Stability
- Unopened: Store at 25–30℃, dry and light-protected. Shelf life: 12 months (≥95% activity retention)
- After opening: Tighten cap, continue storage at 25–30℃, dry and light-protected. Shelf life: 2 months
Primer Design Guidelines
- Length: 28–35 nt (longer than PCR primers)
- Too short (<28 nt): reduced recombinase binding efficiency
- Too long (>35 nt): secondary structures (hairpins, dimers) interfere with specificity
- Concentration: optimize via gradient experiments (0.2–0.6 μM)
- Amplicon length: 80–500 bp recommended
- GC content: 40–60% preferred
- Verify specificity via BLAST to avoid cross-reactivity