Cat. No. JY0307
Product Overview
LF-detect Single-system dual-target CRISPR Lateral flow strip for simultaneous detection of CRISPR enzyme cleavage products and amplification products.
Working Principle
This product uses lateral flow dual-antibody sandwich assay to simultaneously detect target gene CRISPR enzyme cleavage products and LAMP, RAA, RPA, or PCR amplification products.
When CRISPR signal probe (A) and amplification product (B) are modified as follows, this product can be used for detection:
- CRISPR signal probe (A): Biotin at one end and FITC or 6-FAM at the other end
- Amplification product (B): Digoxin (Dig) at one end and Rhodamine (TAMRA) at the other end
Intended Use
Simultaneous detection of CRISPR single-cleavage products and amplification products.
Package Specification
- 50 strips/tube, aluminum foil bag moisture-proof packaging
Storage Conditions and Shelf Life
- Storage: Store in a dark and dry place at 4-30°C
- Shelf Life: 12 months
Procedure
- Take out the corresponding number of test strips according to the number of samples to be tested, and mark them on the absorbent pad. Each test strip can only be used for single detection of 2 different products. When the CRISPR single-cleavage product volume is 50-100 μL, the nucleic acid product can be directly detected in a 200 μL PCR reaction tube. When the product volume is less than 50 μL, ultrapure water needs to be added to make up the volume to 50 μL, mix thoroughly by pipetting, and then detection can be performed.
- After the CRISPR enzyme cleavage reaction of LAMP, RAA/RPA, or PCR amplification products is completed, open the PCR reaction tube and insert the conjugate pad end (arrow end) of the test strip into the PCR reaction tube. The liquid level must not exceed the top of the conjugate pad. Wait for the reading zone to be fully wetted (approximately 1-2 minutes). After the test line (T line) develops color, the test strip can be removed.
- Observe the results within 10 minutes after the control line (C line) develops color. Reading after 10 minutes is invalid.
- Record the detection results and seal and discard the test strips in a safe place.