Cat. No. JY0208
Product Overview
LF-detect Independent Dual-Target lateral flow strips for simultaneous detection of two different nucleic acid amplification products.
Working Principle
This product uses lateral flow dual-antibody sandwich assay for rapid detection of nucleic acid amplification products. Compared with traditional agarose gel electrophoresis detection, the nucleic acid detection test strip is simple to operate, rapid to interpret, contains no toxic substances, and requires no instruments or equipment.
Users only need to label one primer/probe with Biotin and the other primer/probe with FITC or 6-FAM during the first detection sequence primer design, and label one primer/probe with FAM and the other primer/probe with Digoxin during the second detection sequence primer design.
Labeling Scheme: 5’Biotin—-5’FAM(FITC), 5’Digoxin—–5’Biotin
Intended Use
Detection of nucleic acid amplification products.
Package Specification
- 10 strips/package × 5, aluminum foil bag moisture-proof packaging
Storage Conditions and Shelf Life
- Storage: Store in a dark and dry place at 4-30°C
- Shelf Life: 12 months
Procedure
- Take out the corresponding number of test strips according to the number of samples to be tested, and mark them on the absorbent pad. Each test strip can only be used for single detection of a single sample. When the amplification product volume is 50-100 μL, the nucleic acid product can be directly detected in a 200 μL PCR reaction tube. When the product volume is less than 50 μL, ultrapure water needs to be added to the PCR tube to make up the volume to 50 μL, mix thoroughly by pipetting, and then detection can be performed.
- After PCR, RPA, or RAA reaction is completed, open the PCR reaction tube and insert the conjugate pad end (arrow end) of the test strip into the PCR reaction tube. The liquid level must not exceed the top of the conjugate pad. Wait for the reading zone to be fully wetted (approximately 1-2 minutes; when the ambient temperature is low, such as in winter, the wicking speed will be reduced and the wetting time of the reading zone will be extended). After the control line (C line) develops color, the test strip can be removed. Read the detection result directly according to the color development of the test strip.
- Observe the results within 10 minutes after the control line (C line) develops color. Reading after 10 minutes is invalid.
- Record the detection results and seal and discard the test strips in a safe place.