Cat. No. BT0069
Product Overview
Tiosbio® Star Flash Nucleic Acid Release Reagent is a rapid room-temperature nucleic acid extraction reagent optimized for saliva, swabs, and tissue samples.
Characteristics
- This reagent can be used for nucleic acid (both DNA and RNA) extraction, enrichment, and purification from animal samples including saliva, oral swabs, nasopharyngeal swabs, cell suspensions, and tissue blocks, or plant samples including roots, stems, leaves, flowers, and seeds. The product after lysate treatment can be directly used for isothermal amplification with Tiosbio® RAA Nucleic Acid Amplification Kit (JY0200), Tiosbio® RT RAA Nucleic Acid Amplification Kit (JY0203), and Tiosbio® RAA Nucleic Acid Amplification Kit (Lateral Flow Strip Method) (JY0202).
- No addition of enzymatic preparations such as lysozyme, lysostaphin, or proteinase K is required. No use of harmful chemical reagents such as phenol or chloroform is needed.
- One-step, single-tube operation that is convenient and fast, with an operation time of less than 1 minute.
- High sensitivity and good reproducibility. After processing with this kit, taking 1-15 μL of lysate supernatant as template for PCR or RAA/RPA can stably amplify target gene sequences of 10¹ copies or more in the system.
- Can be used for sample pretreatment in food safety, disease control, emerging infectious diseases, animal epidemics, molecular diagnostics, and genetic testing for precision medicine.
Storage Conditions and Shelf Life
- Shelf Life: 12 months
- Specifications: 1 mL per tube
- Storage: Store at room temperature
Procedure
1. Animal Samples
After appropriate processing of samples such as saliva, oral swabs, nasopharyngeal swabs, and cell suspensions, mix with the sample to be tested at a ratio of Star Flash Nucleic Acid Release Reagent (BT0069) to liquid sample = 1:4. For tissue samples, lyse at a ratio of 200 μL lysis solution per 100-200 mg of sample. Crush the material to be tested with a pipette tip for thorough grinding and fragmentation of the sample, then blow and aspirate the lysis solution several times with a pipette tip or disposable plastic pipette, or vortex to mix the solution.
Blow and aspirate the lysis solution several times with a pipette tip or disposable plastic pipette, or vortex to mix the solution, centrifuge briefly, and aspirate the supernatant into a clean centrifuge tube for testing.
For RAA or RT RAA amplification, the lysate supernatant can be added up to the maximum allowable volume of the reaction system.
2. Plant Samples
Cut the material to be tested into 5 mm square small pieces or segments, add 50 μL of Star Flash Nucleic Acid Release Reagent (BT0069), and crush the material with a pipette tip. After thorough grinding and fragmentation of the sample, blow and aspirate the lysis solution several times with a pipette tip or disposable plastic pipette, or vortex to mix the solution. After brief centrifugation of the lysis solution, aspirate the supernatant into a clean centrifuge tube for testing.
For RAA or RT RAA amplification, 2 μL of lysate supernatant can be taken for detection.
Warnings and Precautions
- When using this reagent for nucleic acid extraction from saliva, oral swabs, nasopharyngeal swabs, or cell suspensions, sample processing and amplification should be completed immediately after sample collection whenever possible. If conditions do not allow, samples should be transported under refrigerated or frozen conditions.
- When used for infectious disease sample testing, pay attention to safety protection during operation.