Two-step PCR, omitting the traditional 72°C extension phase and merging it with the 55°C annealing phase, combined with microfluidic rapid thermal cycling, is setting a new gold standard for point-of-care infectious disease testing.
This breakthrough technology means patients no longer wait days or hours for lab results. A Rapid Diagnostic Test (RDT) using two-step microfluidic PCR delivers highly sensitive quantitative data in just 15 minutes. From primary care clinics to global health initiatives, this speed is fundamentally changing the landscape of IVD.