Pushing the Limit of Detection (LOD): How to Achieve Picogram Sensitivity in LFA

The Sensitivity Barrier

Standard lateral flow assays often hit a sensitivity wall at the nanogram level. For early disease detection (e.g., HIV p24, Troponin I), you need picogram (pg/mL) sensitivity.

Enhancement Strategies

Using Due Bio High-Sensitivity Nitrocellulose with a slower wicking rate allows more time for antigen-antibody interaction. Combined with signal enhancement techniques like silver staining or enzyme amplification, you can push the LOD down by 10-50x.