Optimizing Lysis Buffers for Plant Pathogen Detection

Breaking the Cell Wall

Detecting viruses or bacteria in plant leaves (e.g., TMV, Xylella) requires breaking robust cellulose walls. Standard buffers often fail.

High-Salt & Detergent

We recommend a lysis buffer containing PVP (Polyvinylpyrrolidone) to bind plant polyphenols, along with a non-ionic detergent. Due Bio’s “Ag-Grade” sample pads are pre-buffered to neutralize acidic plant saps (like grape or citrus), protecting antibody functionality.

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