Troubleshooting Invalid Tests: Why Does the Control Line Fail?

No C-Line = No Result

A missing Control (C) line renders the test invalid. Common causes include:

  • Conjugate Death: Gold nanoparticles aggregated or antibodies denatured.
  • Membrane Dry-out: Old strips stored without desiccants.
  • Flow Obstruction: Sample pad clogging preventing liquid from reaching the C-line.

Switching to Due Bio robustly packaged and stabilized strips can reduce invalid rates by >90% in field settings.

TL
Global Agent · Duebio (TiosBio) · 20+ Years in IVD
IVD industry veteran specializing in CRISPR Cas12/Cas13 detection, RAA isothermal amplification, lateral flow assays, microfluidic PCR, TRF immunoassays, and OEM/ODM IVD development for global distributors. Duebio is the international trade brand of TiosBio, a Chinese IVD manufacturer with 20+ years of experience.

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