Competitive vs. Sandwich
Unlike COVID-19 (sandwich assay), detecting small molecules like Glyphosate or Antibiotics requires a Competitive Assay format. Here, the T-line intensity decreases as analyte concentration increases.
Critical Tuning
Competitive assays are highly sensitive to membrane flow rate. A slower wicking speed (e.g., 180s/4cm) is crucial to allow sufficient competition at the test line. Due Bio offers defined “Slow-Flow” strips specifically for this application.