Flow Rate vs. Surface Area
Smaller pores (e.g., 8μm) offer higher surface area for antibody binding and slower flow, increasing sensitivity. Larger pores (12-15μm) allow faster flow but reduce reaction time.
Selection Strategy
For high-sensitivity CRISPR assays, we typically recommend 10μm or 12μm membranes as the optimal balance. Due Bio provides a range of defined pore sizes to tune your assay performance.