💡 Application Note: DNA extraction-free multiplex LFA. Compatible with Due Bio’s Multiplex Universal Strips for food safety applications.
Title: Meat Authenticity Made Easy: DNA Extraction-Free Rapid Onsite Detection of Duck and Pork Ingredients in Beef and Lamb Using Dual-Recombinase-Aided Amplification and Multiplex Lateral Flow Strips
Source: Journal of Agricultural and Food Chemistry (ACS) (2023)
Abstract
Meat adulteration is a major global concern that poses a threat to public health and consumer rights. In this study, we developed a rapid onsite identification method for animal-derived ingredients by utilizing a fast nucleic acid lysis buffer to expedite the release of sample nucleic acids and combined it with dual-recombinase-aided amplification (dual-RAA) technology and visual multiplex lateral flow strips (MLFSs). Our method successfully detected duck- and bovine-derived, porcine- and bovine-derived, duck- and ovine-derived, and porcine- and ovine-derived meat in a rapid 20 min onsite detection assay, with a detection limit of 10^1 copies/50 μL reaction system.