💡 Application Note: Digital CRISPR/Cas13a assay. This ultrasensitive diagnostic method is compatible with Due Bio’s RNA detection strip series.
Title: Development and characterization of a digital CRISPR/Cas13a based assay for rapid and sensitive diagnosis of severe fever with thrombocytopenia syndrome virus
Source: Sensors and Actuators B: Chemical (Elsevier) (2023)
Abstract
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a re-emerging pathogen causing sporadic outbreaks. There are currently no licensed vaccines or therapeutics, and early diagnosis of infected cases is critical. Here, we developed three candidate diagnostic assays for field or clinical detection of SFTSV infections. The two-step CRISPR/Cas13a based assay was found to be comparable in sensitivity to previously published work, whereas optimization of reaction conditions for the one-pot CRISPR/Cas13a based assay improved the sensitivity by 40-fold.