Title: A reverse transcription-cross-priming amplification method with lateral flow dipstick assay for the rapid detection of Bean pod mottle virus
Source: Scientific Reports (Nature)
Introduction
Bean pod mottle virus (BPMV) is a comovirus that has a bipartite positive-strand RNA genome consisting of RNA-1 and RNA-2. It has become one of the most economically important soybean viruses in the U.S. In this study, we aimed to establish an RT-CPA-LFD method combined with reverse transcription (RT) to amplify BPMV RNAs and evaluate the potential of this method for detecting BPMV in soybeans.
Key Method (Tiosbio Usage)
“The lateral flow dipstick (LFD) of Tiosbio® LF-detect (Beijing Baoying Tonghui Biotechnology Co., Ltd.) with fluorescein isothiocyanate (FITC) and biotin was used to detect the RT-CPA amplicons. The sample immersion area of the Tiosbio® LF-detection dipsticks was inserted directly into the RT-CPA solution without dilution and incubated for 2 min in an upright position.”