This product uses a chromatographic double-antibody sandwich method to detect the enzyme cleavage products of Cas12 and Cas13. After the target gene is amplified by LAMP, RAA/RPA, and PCR, use Cas12 and Cas13 enzymes to cut the amplified product at the same time, and the DNA signal probe A corresponding to the Cas12 enzyme (Cas12 cleavage), or the RNA signal probe corresponding to the Cas13 enzyme Needle B (Cas13 cleavage) is modified as follows, then this product can be used for detection of cleavage products:
- One end of probe A is modified with biotin (Biotin), the other end is modified with fluorescein isothiocyanate (FITC) or 6-carboxyfluorescein (6-FAM), and one end of probe B is modified with biotin (Biotin) , the other end is modified with digoxin (Dig);
- Or, one end of probe A is modified with biotin (Biotin), the other end is modified with digoxin (Dig), and one end of probe B is modified with biotin (Biotin), and the other end is modified with fluorescein isothiocyanate ( FITC) or 6-carboxyfluorescein (6-FAM).